- Generation of custom-made viral plasmid vectors (planning and cloning of new viral vectors)
- Virus Production
- Virus Quality Control (quantification and testing)
Recombinant adeno-associated viral particle (rAAV) (Prof. Dr. S. Schoch McGovern, Dr. K. van Loo)
To generate recombinant adeno-associated viruses different shuttle vector types can be used.
Available promoters: CMV, hSynapsin I, EF-1 alpha, CAG (CMV early enhancer/chicken beta actin), CamKII
- Constitutive expression
- Conditional expression (Cre-recombinase, Flp-recombinase)
- Containing fluorescent marker proteins (as reporter, as fusion protein, separated by the 2A peptide sequence, or after an IRES sequence)
Serotypes: Depending on the target cell type different serotypes of rAAV can be purified.