Viral vector service – Cloning
Adeno-associated virus (AAV) and Lentivirus (LV) can be generated and cloned upon request. We use standard cloning techniques; restriction-ligation approaches and Infusion Cloning. Vector for viral transfection will be made using the Endofree Qiagen Kit. All newly made plasmid DNAs will be sequenced and tested for fluorescence activity.
Viral vector service – Purification AAV
For AAV replication we use helper plasmids that provide all the necessary adenoviral genes.
Depending on the target cell type different serotypes of rAAV can be purified(serotyp 1,2,5,8,DJ8,9) . For the purification of new AAVs we can used heparin columns or Iodixanol gradient centrifugation.
– Generation of endotoxinfree plasmid DNA for viral production
– Cloning of new AAV or LV vector (simple or complex cloning strategies)
– AAV purification (Heparin columns or gradient centrifugation)
– SDS-Page Coomassie staining and viral transduction test in primary neurons
Virus-Shop coming soon