Next Generation Sequencing

The mission of the Next Generation Sequencing (NGS) Core Facility of the Medical Faculty of the University of Bonn is to provide the research community with access to state-of-the art sequencing technologies and to help facilitate cutting-edge-omics research. We offer rapid high-throughput sequencing services, consultation and expert assistance in strategic planning, sample preparation and method development.

Due to ongoing technical advancements NGS is widely applied in many research projects. But, despite decreasing sequencing costs during the last years, the access to this technology is made difficult as investment in and maintenance of NGS systems as well as the establishment of research specific applications remains expensive and time consuming. Therefore, the NGS Core Facility, which is allocated at the Institute of Human Genetics in Bonn, provides assistance and technical equipment to all scientist at the University of Bonn and collaborating institutions. This service is all-encompassing as it is intended to cover all phases of your project. Therefore, researchers are encouraged to contact the NGS Core Facility for consultation prior to sequencing projects, which will allow us to provide initial support in study design and therefore optimize the performance of the NGS experiments. The suitability of NGS technology is diverse – like sequencing of entire exomes or genomes, transcriptomics (coding/non-coding RNA), epigenomics, metagenomics (16S rRNA) and expression analysis by 3´-mRNA sequencing. Additionally, we are offering deep-sequencing of amplicons (i.e. for genotyping of CRISP-Cas9 generated clones). Furthermore, NGS is indispensable for de novo sequencing of human pathogenic or resistance-relevant bacteria and viruses, resequencing of disease related genomic regions and ChIP sequencing.

 

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