The Alpaca Nanobody Core Facility offers the customized generation of alpaca variable domains of heavy chain-only antibodies (VHHs), also called nanobodies, against target structures of choice. These highly specific single domain antibodies (ca. 15 kDa) recognize their targets with affinities comparable to conventional antibodies. Yet, we can identify suitable nanobodies by phage display, produce them in bacteria, or express them in the cytosol of mammalian cells to visualize targets or alter protein function. The ease of modification by genetic fusion or chemical/enzymatic conjugation makes them versatile tools for perturbation studies, microscopy, flow cytometry, mass cytometry, mass spectrometry, or non-invasive imaging methods such as immuno-PET. Nanobodies also facilitate protein crystallization and are widely used in structural biology. We offer the immunization of alpacas with provided protein, as well as the identification and validation of specific nanobodies against your desired target. We will help you with the production and (site-specific) modification of nanobodies to adjust them to your needs.