Instrumentation

autoflexMALDI-TOF/TOF Bruker Autoflex III

Our MALDI-TOF instrument is a versatile tool for fast analyses of proteins and peptides. Low complexity protein samples such as from 2D gel spots can be analyzed with good mass accuracy and resolution. The TOF detector can also be used for intact protein mass measurements and fast characterization of protein preparations.

Ion trap Bruker HCT Ultra and HCT Ultra/PTM

hct1These two spectrometers use electrospray ionization techniques and are usually coupled to liquid chromatography systems (micro- or nanoflow). They offer good performance for analyses of medium complex protein samples. MSn experiments can be performed in the ion traps for more detailed manual examination of analytes. One of the instruments offers electron transfer dissociation (ETD) for gentle fragmentation of posttranslationally modified peptides (e.g. phosphopeptides). Hybrid instrument

orbitrapThermo Orbitrap Velos

The Orbitrap Velos has two detectors: a fast low resolution linear ion trap and a high resolution, high accuracy Orbitrap detector which can be used in parallel. This instrument is our workhorse for in-depth proteome analyses and whenever high resolution and sensitivity are needed.

nanoLC systems

lcsagilentTwo ultra-high performance liquid chromatographs for nanoflow (~300 nl/min) are used for coupling with mass spectrometers. These systems perform chromatographic separations very reproducibly and assure a stable delivery of analytes to the mass spectrometers. An Advion Triversa Nanomate nanospray robot can be used for automated delivery of small sample amounts for direct infusion.
Sample preparation devices can be provided to handle larger sets of samples with the help of a gel imaging/spot cutting device, a pipetting robot, and a pI-based peptide fractionation