Instrumentation

Thermo Orbitrap Tribrid Fusion Lumos

Lumos

ThermoScientific Orbitrap Fusion Lumos

The „Lumos“ is our new flagship: A state-of-the-art hybrid instrument that has a quadrupole, Orbitrap, and ion-trap mass analyzers. In combination with three different fragmentation methods (including electron transfer dissociation – ETD) it is probably the most versatile instrument available. All high throughput projects will benefit from its improved sensitivity and speed. New measurement modes will be available: analyses of intact proteoforms, data-independent and targeted techniques (MRM / PRM).

Thermo Orbitrap Velos

Velos

ThermoScientific Orbitrap Velos

The Orbitrap Velos has two detectors: a fast low resolution linear ion trap and a high resolution, high accuracy Orbitrap detector which can be used in parallel. This instrument is our workhorse for proteome analyses and whenever higher resolution and sensitivity are needed.

MALDI-TOF/TOF Bruker Autoflex III

MALDI

Bruker Autoflex III MALDI-TOF

Our MALDI-TOF instrument is a versatile tool for fast analyses of proteins and peptides. Low complexity protein samples such as from 2D gel spots can be analyzed with good mass accuracy and resolution. The TOF detector can also be used for intact protein mass measurements and fast characterization of protein preparations.

Ion trap Bruker HCT Ultra

Ion Trap

Bruker HCTultra Ion Trap

This ion trap spectrometer uses electrospray ionization and is usually coupled to liquid chromatography systems (micro- or nanoflow). It offers good performance for analyses of medium complex protein samples. MSn experiments can be performed in the ion trap for more detailed manual examination of analytes.

nanoLC systems

lcsagilentTwo ultra-high performance liquid chromatographs for nanoflow (~300 nl/min) are used for coupling with mass spectrometers. These systems perform chromatographic separations very reproducibly and assure a stable delivery of analytes to the mass spectrometers. An Advion Triversa Nanomate nanospray robot can be used for automated delivery of small sample amounts for direct infusion.
Sample preparation devices can be provided to handle larger sets of samples with the help of a gel imaging/spot cutting device, a pipetting robot, and a pI-based peptide fractionation