Instrumentation

Location: BMZ

Zeiss Observer.Z1 wide-field fluorescent microscope

The Zeiss microscope is an easy-to-use instrument, equipped with strong halogen-lamp and multi-line LED light-source. The instrument also has multiple filter sets for the most common fluorophores: DAPI, CFP, GFP, YFP, RFP, Cy5 and Cy7. Additionally, polarization filters can be used for imaging crystalline materials. The dual-cam system also allows for ratiometric imaging of FRET-sensors and for fast processes, like calcium-spikes. The instrument is also capable of live imaging from multi-well plates with proper temperature-, CO2– and O2-control, even in hypoxic conditions. New addition to the instrument is a full environmental chamber and the ApoTome.2 system for optical sectioning of thick samples.

Leica SP5 AOBS with SMD confocal microscope

The Leica AOBS confocal microscope line provides the strongest illumination in any confocal system due to its nonlinear optics. The microscope is also completely without filters, which gives the highest flexibility for selecting the best light paths matching your fluorophores. The instrument is equipped with multi-line Argon (457, 474, 488, 497 and 514 nm), 561 nm DPSS and 633 nm HeNe lasers. Additionally, the Single Molecule Detection (SMD) unit provides pulsed laser lines at 405, 470 and 640 nm, and two Single Photon Avalanche Diode (SPAD) detectors for fluorescence lifetime (FLIM) and fluorescence correlation spectroscopy (FCS) measurements. The instrument is equipped with full environmental chamber.

Location: Life&Brain research center

Leica SP8 AOBS confocal microscope

The Leica AOBS confocal microscope line provides the strongest illumination in any confocal system due to its nonlinear optics. The microscope is also completely without filters, which gives the highest flexibility for selecting the best light paths matching your fluorophores. The instrument is equipped with 405, 488, 552 and 640 nm solid state lasers, and the latest generation HyD detectors. The upright confocal system is set up for imaging large tissue sections and cleared whole brain samples. An additional SMD unit is planned for FLIM measurements.