Instrumentation

Location: BMZ

Zeiss Observer.Z1 wide-field fluorescent microscope

The Zeiss microscope is an easy-to-use instrument, equipped with strong halogen-lamp and multi-line LED light-source. The instrument also has multiple filter sets for the most common fluorophores: DAPI, CFP, GFP, YFP, RFP, Cy5 and Cy7. Additionally, polarization filters can be used for imaging crystalline materials. The dual-cam system also allows for ratiometric imaging of FRET-sensors and for fast processes, like calcium-spikes. The instrument is also capable of live imaging from multi-well plates with proper temperature-, CO2– and O2-control, even in hypoxic conditions. New addition to the instrument is a full environmental chamber and the ApoTome.2 system for optical sectioning of thick samples.

Leica SP5 AOBS with SMD confocal microscope

The Leica AOBS confocal microscope line provides the strongest illumination in any confocal system due to its nonlinear optics. The microscope is also completely without filters, which gives the highest flexibility for selecting the best light paths matching your fluorophores. The instrument is equipped with multi-line Argon (457, 474, 488, 497 and 514 nm), 561 nm DPSS and 633 nm HeNe lasers. Additionally, the Single Molecule Detection (SMD) unit provides pulsed laser lines at 405, 470 and 640 nm, and two Single Photon Avalanche Diode (SPAD) detectors for fluorescence lifetime (FLIM) and fluorescence correlation spectroscopy (FCS) measurements. The instrument is equipped with full environmental chamber.

Location: Life&Brain research center

Leica SP8 AOBS confocal microscope

The Leica AOBS confocal microscope line provides the strongest illumination in any confocal system due to its nonlinear optics. The microscope is also completely without filters, which gives the highest flexibility for selecting the best light paths matching your fluorophores. The instrument is equipped with 405, 488, 561 and 640 nm solid state lasers, and the latest generation HyD detectors. The upright confocal system is set up for imaging large tissue sections and cleared whole brain samples. An additional SMD unit is planned for FLIM measurements.

Light-sheet fluorescence microscope (LSFM)

A home-built light sheet microscope was designed for fluorescence recording of optically cleared specimens (e.g. whole mouse brain) for both generation of rapid overviews and high resolution Z-stack mosaic datasets. It covers a wide magnification range by selected combinations of excitation objectives, detection objectives and detection tube lenses. The instrument is equipped with 488 and 561 nm lasers, and an Orca Flash 4 CMOS camera. Image acquisition is controlled via a Labview program. The instrument is currently not available, but it will be possible to book at a later time.

Location: Haus für Experimentelle Therapie

Zeiss LSM 780 upright confocal microscope

The upright Examiner instrument is equipped with multiple laser lines (405, 458, 488, 514, 543, 594 and 633 nm) as well as with a tuneable and a fixed 2-photon fiber lasers. Additionally, the instrument is equipped with a spectral detector that allows for spectral unmixing and four highly sensitive GaAsP Dectectors (BiG) for optimal emission detection. The microscope is further encased by an environmental chamber allowing for temperature control. Together with an isofluran anesthesia system, this instrument is optimally set-up for intravital microscopy of live animals. Multiple filter sets to separate e.g. CFP, GFP, YFP, tdTomato, SHG and fluorescent dyes are available. Our protocols and motorized stages allow for intravital imaging of various organs.