One-Day Seminar: Flow Cytometry in Immunology, 24th February

If you are looking for new ideas and strategies or if you just want to refresh your knowledge about immunological methods in flow cytometry, consider to take part in a one day seminar “Flow Cytometry in Immunology” on the Campus Venusberg (How to find us .pdf).

Time: Tuesday, 24th of February

9:00-16:00 (lunch break 12:00-13:00)

Location: Biomedizinisches Zentrum, Campus Venusberg, kleiner Hörsaal

And we are happy to have Manfred Kubbies as an expert on site, who has more than three decades experience in academic and industrial research and who will guide you through the various topics in flow cytometry.

What you will hear about:

Immunology

  • Antibodies: Polyclonal and monoclonal antibodies: structure, function, binding, crossreactivity, affinity, AB fragments
  • Stain no wash …or… stain and wash: unspecific AB binding, unbound fluorochromes, FcR binding
  • Fluorochromes for AB immunolabeling: single- and multicolor, Multicolor fluorescence crosstalk: compensation
  • AB binding sites quantification
  • CD clusters and designation
  • Morphology and scatter of hematopoietic cells
  • T/B receptor variation during ontogeny; reference values
  • Activation dependent receptor modulation
  • Subpopulation specific cell cycle analysis
  • The problem of soluble/shedded receptors in cell analysis
  • Receptor internalization analysis techniques: a cheap inside/outside analysis
  • Cellular AB phosphoprotein analysis
  • Detection of intracellular cytokines and cytokine secretion

Analysis: Scales, means and thresholds

  • Linear vs logarithmic vs bi-exponential scale recording
  • Don´t trust your eyes on log scale recorded data
  • Median, arithmetic or geometric mean
  • Threshold, gate, region: artifical or knowledge based decision

The speaker:

Manfred_KubbiesManfred Kubbies build his first “Impulscytophotometer” back in 1977. He then studied Biology at the Unversity of Würzburg, where he finished his PhD thesis at the Institute of Genetics, on cellular aging and cell proliferation, as measured by flow cytometry. He then became Professor and continued working at the University of Würzburg. He left the university in the late 1980s to work for Roche on cellular and genetic therapies for cancer, where he still kept his curiosity for new analytical methods on a cellular level. He is an experienced instructor and he can combine the knowledge from “the good old days” in flow cytometry with modern cytometric cell analysis, and the challenges we are facing today.

Registration:

We would like to ask you to register for the Seminar, so that we can plan the lunch break. And we would like to give us some idea of your education level, to tailor the topics of the seminar to your needs.

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