The Cell Programming Core Facility employs state-of-the-art cell (re-)programming technologies and is constantly refining its technological portfolio. To promote the establishment of innovative stem cell-based in vitro models we offer services on the generation and provision of human iPS cell lines.
As starting material for iPS cell derivation we accept skin fibroblasts or peripheral blood mononuclear cells (PBMCs). Donor cells are reprogrammed using the latest Sendai virus-based reprogramming vectors that guarantee transient introduction of transgenes to avoid the stable integration of reprogramming factors. As a standard procedure, we isolate and expand up to five clonal iPS cell lines per subject for cryopreservation and subsequent shipping to the investigator. Depending on the recipients’ demands, selected clones are subjected to standardized quality control regimens (ICC and gene expression profiling for pluripotency analysis, assessment of transgene removal, STR analysis, SNP genotyping). Please consult our detailed price list for current rates.
For services in the context of neurological research aims please contact the Cell Programming Unit, a joint platform founded by the University of Bonn Medical Faculty and the German Center for Neurodegenerative Diseases (DZNE).